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1 March 2007 Critical Evaluation of Quantitative Sampling Methods for Aedes aegypti (Diptera: Culicidae) Immatures in Water Storage Containers in Vietnam
Tessa B. Knox, Nguyen Thi Yen, Vu Sinh Nam, Michelle L. Gatton, Brian H. Kay, Peter A. Ryan
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Abstract

In response to an identified paucity of information on the size and composition of immature Aedes aegypti (L.) (Diptera: Culicidae) populations in large field containers, we assessed net sampling and pumping/sieving methods for estimating and enumerating third (III)/fourth (IV) instar and pupal populations. Sweep net detection thresholds (number above which ≥90% chance of a positive sample) were ≤28 immatures for seven different container types (115–3000 liter jars and tanks) in the laboratory, and mean recovery percentages varied by container type (6.15–41.29 and 7.39–33.10% for III/IV instars and pupae, respectively). A pumping method or hand bailing was applied in the field for the collection of III/IV instars and pupae from 406 receptacles, of which 343 had been previously sampled via a five-sweep netting technique. Larvae were 9.30 times more prevalent than pupae, and abundance varied by container type with means of 36–537 III/IV instars and 6–53 pupae per receptacle. Sweep netting for III/IV instars effectively identified 86.2% of Ae. aegypti-positive containers, whereas sampling for pupae detected only 43.1% of positive containers. When conversion factors (inverse of laboratory recovery percentages) were applied to field net sampling data, estimates of container populations were more accurate for III/IV instars than pupae (maximum R2 = 0.610 and 0.328, respectively); however, the relationship between immature abundance and emergent adult populations remains to be defined.

Tessa B. Knox, Nguyen Thi Yen, Vu Sinh Nam, Michelle L. Gatton, Brian H. Kay, and Peter A. Ryan "Critical Evaluation of Quantitative Sampling Methods for Aedes aegypti (Diptera: Culicidae) Immatures in Water Storage Containers in Vietnam," Journal of Medical Entomology 44(2), 192-204, (1 March 2007). https://doi.org/10.1603/0022-2585(2007)44[192:CEOQSM]2.0.CO;2
Received: 16 June 2006; Accepted: 19 November 2006; Published: 1 March 2007
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KEYWORDS
abundance
Aedes aegypti
net
sampling
surveillance
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